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. 2014 May 29;2014:536049. doi: 10.1155/2014/536049

Figure 2.

Figure 2

Flow cytometry analysis of LNCaP cells using PI staining. Time- and dose-dependent effects of (a) TNF-α (10 and 100 ng/mL), (b) IL-13 (20 and 100 ng/mL), (c) IL-1β (2 and 5 ng/mL), (d) IFN-γ (25 and 50 ng/mL) on LNCaP cell death (sub-G1 fraction) after treatment for 24 h or 72 h (*P < 0.05), and (e) representative histograms of cell distribution according to their DNA content, as determined by flow cytometry using PI staining (M1 represents G1 phase, M2 S phase, M3 G2/S phase, and M4 sub-G1 fraction).