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Immunostaining for F4/80 (macrophages) and Ly6G+ (neutrophils) in colon tissue sections. (a, c) GF-DSS and (b, d) BF-DSS. (e) The numbers of F4/80- and Ly-6G+-positive cells were counted in the GF-DSS and BF-DSS group (n = 3 per each group). *P < 0.05 compared with GF-DSS. H&E, magnification ×200, Bar = 20 μm.
