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. 2014 May 25;2014:724670. doi: 10.1155/2014/724670

Figure 2.

Figure 2

Effects of lycopene on U-II-induced ROS production in cardiomyocytes. (a) ROS levels after adding lycopene or the NAD(P)H oxidase-4 (NOX-4) inhibitor apocynin in cells exposed to U-II for 2 min. The ROS levels of cells pretreated for 6 h with lycopene (3 μM) or 30 min with apocynin (0.3 mM) and exposed to U-II (1 nM) for 2 min. (b) Upper panels: representative western blot analysis of NOX-4 in cells pretreated for 6 h with lycopene (3 μM) then exposed to U-II. Lower panels: the indicated values represent the ratio of NOX-4 to GAPDH. The results are shown as mean ± SEM (n = 6).*P < 0.05 versus control.