Table 1.
Primers used for qPCR validation.
| Gene | Primer sequence (5′-3′) | Target size (bp) | Tm (°C) |
|---|---|---|---|
| Actin, beta | Forward: CTGTCGAGTCGCGTCCACCC Reverse: ACATGCCGGAGCCGTTGTCG |
128 |
59 |
|
| |||
| Cyp11a1 | Forward: AGATCCCTTCCCCTGGTGACAATG Reverse: CGCATGAGAAGAGTATCGACGCATC |
192 |
60 |
|
| |||
| Cyp17a1 | Forward: CCAGGACCCAAGTGTGTTCT Reverse: CCTGATACGAAGCACTTCTCG |
250 | 59 |
|
| |||
| Prm2 | Forward: CAAGAGGCGTCGGTCA Reverse: TGGCTCCAGGCAGAATG |
167 | 59 |
|
| |||
| Tex15 | Forward: ATTTGAGTGGCACAGAC Reverse: AGTATTGGGATTTGGAG |
194 | 59 |
|
| |||
| Adam9 | Forward: CGCTTAGCAAACTACCTG Reverse: TTCCCGCCACTGAACAA |
147 | 59 |
|
| |||
| Dazl | Forward: GGAGGCCAGCACTCAGTCTTC Reverse: AGCCCTTCGACACACCAGTTC |
184 | 60 |