Table 1.
HBV specific MHC class I restricted peptides identified by immunoproteomics. The LC-MS/MS selection criteria for peptides are as follows: Charge 1: Xcorr > 1.6; Charge 2: Xcorr > 1.8; Charge 3: Xcorr > 2.0. The HLA binding scores were calculated using the SYFPEITHI prediction program [28].
| Peptide sequence | Protein | Accession ID | HLA motif | m/z | Charge | Xcorr | HLA binding score |
|---|---|---|---|---|---|---|---|
| GGPNLDNIL | Large E | Q8QSF2 | A2 | 457 | 2 | 1.88 | 13 |
| LTFGRETVLEN | Precore/core (C) | Q6UFV9 | A2 | 640 | 2 | 2.18 | 19 |
| LTTVPAASLLA | Large E | Q9YKJ7 | A2 | 530 | 2 | 2.12 | 20 |
| ILRSFIPLL | Surface (S) | Q6WYY8 | A2/24 | 537 | 2 | 1.81 | A2: 28; A24: 12 |
| FLKQQYMNL | Polymerase (P) | I0DE20 | A2/24 | 1185 | 1 | 1.92 | A2: 20; A24: 9 |
| FLSKQYMDL | Polymerase (P) | L7QBE1 | A2/A24 | 573 | 2 | 1.80 | A2: 21; A24: 11 |
| TVSTKLCKI | Polymerase (P) | Q8B4E6 | A2/A24 | 497 | 2 | 1.92 | A2: 19; A24: 14 |
| FLGGPPVCL | Surface (S) | Q0EED2 | A2/A24 | 452 | 2 | 2.05 | A2: 26; A24: 11 |