Table 3.

Overview of the multiple conditions used for the various PCR stages

Marker	PCR 1					PCR 2 (touchdown)
	Primers	Conditions				Primers	Conditions
								3×	3×	3×		30×
nITS Trebouxia	18S-ITS-uni-for ITS4T	D	95°	00:30	×35	ITS1aT ITS4bT	D	95°	95°	95°	00:30	95°	00:30
		A	56°	00:30			A	56°	55°	54°	00:30	53°	00:20
		E	72°	00:40			E	72°	72°	72°	00:40	72°	00:40
cp-psbL-J Trebouxia	psbF psbR	D	95°	00:30	×35	psbL-sense psbJ-antisense	D	95°	95°	–	00:30	95°	00:30
		A	50°	00:30			A	53°	52°	–	00:30	51°	00:20
		E	72°	00:50			E	72°	72°	–	00:50	72°	00:50
nITS Asterochloris	nr-SSU-1780-5′ ITS4	D	95°	00:30	×35	ITS1-sense-A ITS2-antisense-A	D	95°	00:30	×35
		A	55°	00:40			A	54°	00:30
		E	72°	00:30			E	72°	00:40

Every PCR started with an initial denaturation at 95 °C for 2 min

D denaturation, A annealing, E extension