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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Int J Parasitol. 2013 Feb 19;43(6):439–451. doi: 10.1016/j.ijpara.2012.12.012

Fig. 6.

Fig. 6

Anti-platelet aggregation function assay. The effect of recombinant (r) Amblyomma americanum AamAV422 protein (rAamAV422) on platelet aggregation was done using the whole blood approach as described in Section 2.8. Five hundred μL of cattle whole blood mixed 9:1 with sodium citrate diluted with equal volume normal saline (0.9% sodium chloride) was pre-incubated with indicated various amounts of rAamAV422 for 10 min at 37°C. Adding 20 μM ADP triggered platelet aggregation, which was subsequently monitored as a function of electrical resistance using the Chrono-Log aggregometer.