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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Int J Parasitol. 2013 Feb 19;43(6):439–451. doi: 10.1016/j.ijpara.2012.12.012

Fig. 7.

Fig. 7

The effect of recombinant (r) Amblyomma americanum AamAV422 protein (rAamAV422) against plasma clotting in the recalcification time assay. Twenty μL of citrated human plasma diluted up to 90 μL with 10 mM HEPES buffer plus 150 mM NaCl, pH 7.4, was pre-incubated at 37°C for 5 and 10 min with indicated amounts of affinity purified rAamAV422. In the microplate, clotting time was triggered by adding 10 μL of 25 mM CaCl2+ and then monitored as described in Section 2.8.