Amino acid sequence alignment of orthologous tick cystatins showing >50 % amino acid identity: A
I. scapularis cystatin AAY66864 is aligned with metastriata tick cystatin sequences, A. maculatum (AamMacAEO35899), D. variabilis (DmVarACF35512), H. longicornis (HaLoABZ89553), R. sanguineus (RhiSanACX53850) and R. microplus (RHiMiABG369312), B
A. americanum (AEO36092) aligned with H. longicornis (ABZ89554), C
D. variabilis (ACF35514) aligned with A. americanum (AEO35688)