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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Med Epigenet. 2014 Feb 14;2(1):28–36. doi: 10.1159/000358882

Fig. 3.

Fig. 3

Effect of the cycling incubation protocols on conversion efficiency for IL6ST methylation (individual A), Comparison of different DNA incubation protocols (‘cycling 16 h’, ‘cycling 8 h’ and ‘manufacturer’s’, see table 1) in determining IL6ST methylation (%) estimates assessed on human whole-blood DNA from individual A using the Epitect+, MethylDetector, Zymo Gold and Zymo Methylation kits. Data are reported as mean of two different bisulfite treatments after which two technical replicates have been performed (SD across bisulfite and technical replicates was <5% for each of the CpG sites analyzed).