Table 2.
Location and characteristics of pyrosequencing amplicons for ALUSX, IL6ST, IGF2 and ST3GAL2 used inthe study
| Name of assay |
Chromo- some |
Start | End | No. of CpGs analyzed |
Forward | Reverse | Sequencing | Biotiny- lated |
Annealing temperature |
|---|---|---|---|---|---|---|---|---|---|
| ALUSX | _ | _ | _ | 6 | TTTTTATTAAAAA- | CCCAAACTAAAA- | AATAACTAAAA- | Forward | 50° C |
| TATAAAAATT | TACAATAA | TTACAAAC | |||||||
| IGF2 | 11 | 2169369 | 2169402 | 3 | TGAGGATGGGTTTTT- | TCCTCGATCCACCC- | GGGGTGGA- | Reverse | 56° C |
| GTTTGGTAT | AAAATAATAT | GGGTGTA | |||||||
| IL6ST | 5 | 55290797 | 55290852 | 9 | TGAAGGAGTAGGGA- | ACCTCT GCGGAG | TGAAGGAGTAGGG- | Reverse | 54° C |
| TTTGAAGC | AAG GAT CT | ATTTGAAGC | |||||||
| ST3GAL2 | 16 | 70423257 | 70432300 | 5 | GGTTTTTTTGTTGGT- | CTTACCCCAAAACC- | GGGTTTTGG- | Reverse | 55°C |
| GTTTATTATGT | TCTCCTTACTAAA | ATGGGA |
Nucleotide position according to the February 2009 human reference sequence (GRCh37/hgl9) produced by the International Human Genome Sequencing Consortium.