Skip to main content
. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Cancer Res. 2014 Apr 24;74(12):3218–3227. doi: 10.1158/0008-5472.CAN-13-1706

Figure 5. MiR-542-3p activates p53 pathway in MDM2-overexpressing or ARF-deficient cells.

Figure 5

(A) MiR-542-3p increases p53 in two ovarian clear cell carcinoma lines (JHOC7 and OVISE) and one glioma cell line (LN229). Cells were transfected with negative or miR-542-3p mimics followed by western blot analysis. (B) MiR-542-3p inhibits cell growth. Cells were reseeded at 1x104 cells/well in 12-well plates, cultured for 5 days and then fixed for crystal violet staining. Relative cell growth was calculated after re-solubilizing the plates. (C) MiR-542-3p induces G1 cell cycle arrest. Control or miR-542-3p-overexpressing cells were fixed and stained for cell cycle profiling by FACS. (D-F) p53 is crucial for miR-542-3p-induced cell death. HCT116 p53 WT and p53™/™ cells were transfected as indicated followed by western blot (D) or cell cycle analysis (E). Percentage of cells in sub-G1 was quantitated (F). *, P < 0.05. **, P < 0.01.