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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Prog Retin Eye Res. 2014 Apr 4;0:64–89. doi: 10.1016/j.preteyeres.2014.03.002

Table 3.

Comparison of protein and sterol metabolite levels of different cholesterol-metabolizing CYPs in human NR, RPE, and brain

Donor CYP27A1,
fmol/mg
protein		 27-COOHa,
pmol/mg
protein		 CYP46A1,
fmol/mg
protein		 24-OHa,
pmol/mg
protein		 CYP11A1,
fmol/mg
protein		 Pregnenolone,
fmol/mg protein
Human Neural Retina
17 570 ± 51 25b 63 ± 5 2 NDd
20 533 ± 63 51 59 ± 6 3 ND
12 464 ± 38 130 58 ± 7 4 ND
13 509 ± 51 29 59 ± 5 1 6c ND
8 NDd 75 ND 2 3
9 ND 125 ND 1 2
11 ND 37 ND 4 4
Human RPE
17 1,170 ± 200 ND ND ND ND ND
20 1,570 ± 190 ND ND ND ND ND
12 1,460 ± 240 ND ND ND ND ND
13 2,010 ± 180 ND ND ND ND ND
8 ND 2 ND <1e ND
9 ND 10 ND ND <1e
11 ND 3 ND ND
Human Brain (gray matter temporal lobe)
Donor CYP27A1,
fmol/mg
protein		 27-OHa,
pmol/mg
protein		 CYP46A1,
fmol/mg
protein		 24-OH,
pmol/mg
protein		 CYP11A1 Pregnenolone,
fmol/mg protein
1 121 ± 19 ND 385 ± 45 ND ND
2 117 ± 11 22 ± 1 326 ± 35 1,294 ± 42 16 ± 5f 14 ± 2
4 114 ± 16 14 ± 1 356 ± 34 1,339 ± 45 14 ± 1
3 107 ± 19 11 ± 1 381 ± 37 1,160 ± 56 9 ± 1
a

27-COOH, 5-cholestenoic acid; 24-OH, 24-hydroxycholesterol, 27-OH, 27-hydroxycholesterol.

b

Lack of error bars indicates single measurement; all other results represent the mean ± SD of triplicate measurements.

c

Bovine NR.

d

ND, not determined.

e

Limit of detection.

f

Donor 5.

Information sources (Heo et al., 2011; Liao et al., 2011; Liao et al., 2010; Mast et al., 2011; Wang et al., 2012)