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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Neurochem Int. 2014 Feb 24;0:4–15. doi: 10.1016/j.neuint.2014.02.003

Figure 5.

Figure 5

Protection by ligand is not temperature-dependent. The 4 °C- assay was performed just as for RT, except the membranes were kept at 4 °C during the cocaine and MTSEA incubations. The step with [3H]MFZ binding was performed at RT. To compensate for the slower reaction, 2.5-fold higher MTSEA concentrations were used in the 4 °C-assay. Similar protection by cocaine and dopamine at 4 °C was seen for N336C and M342C as well (not shown). Data displayed here are from a single representative experiment performed in triplicate.