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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Neurochem Int. 2014 Feb 24;0:4–15. doi: 10.1016/j.neuint.2014.02.003

Figure 6.

Figure 6

SASA for TM6-IL3-TM7 in the occluded and inward-facing conformations of DAT. SASA values were calculated for each position after residues were mutated to cysteines, one at a time, and then minimized and equilibrated in a water/lipid environment. In the SASA analysis, only surface area accessible to solvents was counted; surface area exposed to lipids was treated as buried. The SASA percentage was obtained as described in Methods. The 8.5 % threshold chosen to define accessibility is indicated with a stippled line.