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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Mol Cancer Res. 2014 Mar 20;12(6):953–964. doi: 10.1158/1541-7786.MCR-13-0668

Figure 6. CXCR4-mediated chemotaxis is dependent on Rac1 and Cdc42.

Figure 6

A. Western blot of CHLA-25 and TC32 cells shows no significant induction of either PERK (left) or P-AKT (right) following 24 hr. exposure of serum starved cells (SFM) to SDF-1α (100ng/mL).

B & C. End-point analysis of cell migration towards SDF-1α in the presence or absence of Rac1 (NSC 23766) or Cdc42 (ML141) inhibitors was performed as described in Materials and Methods using transwell assays and crystal violet staining. Inhibition of Rac1 and Cdc42 both impeded CXCR4-dependent cell migration.

D & E. Pharmacologic inhibition of Rac1 (NSC 23766) and Cdc42 (ML141) inhibits CXCR4-dependent migration (D) and invasion (E) of CHLA-25 and TC32 cells. Summary histograms show mean ± SEM of three independent experiments with four replicates per condition. *, P < 0.05, **, P < 0.01 and ***, P < 0.001 as compared to controls.

F. Rac1 activity was measured in TC32 cells sorted on the basis of CXCR4. Absorbance values are normalized to control condition (0% in CXCR4-low) and summary histograms show mean ± SEM of two independent sorts with three replicates per condition. *, P < 0.05