Figure 5.
Inhibition of contractility prevents PLB retraction but has no effect on extension. The peak extension of PLBs was measured at 0.5 min and peak retraction at 5 min after nsPEF exposure. Note that these timepoints correspond to 150 s and 420 s of recordings, respectively. The data are presented as mean +/− S.E. for the following groups: control (buffer only), vehicle controls (DMSO 0.1 and 0.25%), blebbistatin in 0.1% DMSO and Y-27632 in 0.25% DMSO. The inhibition of PLB contraction by blebbistatin and Y-27632 is statistically significant at p<0.01 (Student’s t-test) as compared to respective vehicle controls.