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. Author manuscript; available in PMC: 2015 Jul 17.
Published in final edited form as: Virus Res. 2014 Jan 8;0:65–71. doi: 10.1016/j.virusres.2013.12.041

Figure 1. Gn and GnT Protein Expression Regulates TBK1 Directed ISRE Promoter Transcription.

Figure 1

A) HEK293T cells were transfected with ISRE promoter directed luciferase reporter and Renilla luciferase plasmids (Matthys et al., 2013). Cells were co-transfected with a TBK1 expressing plasmid and increasing amounts of ANDV, NYV or PHV GnT-C42 expression plasmids. Cells were harvested 1 day post-transfection and assayed for firefly luciferase activity. Results are presented as the percent induction compared to pcDNA3 induction control (100%) after standardization to Renilla luciferase levels as previously described (Matthys et al., 2013). B) HEK293T cells were transfected with IFNβ promoter directed luciferase reporter and Renilla luciferase plasmids and co-transfected with TBK1 plasmid and either ANDV or PHV Gn-T or the ANDV M segment expression plasmid. Cells were harvested and luciferase reporter responses analyzed as in A. ANDV Gc and β-actin (loading control) were detected by Western Blot analysis as described (Matthys et al., 2013).