. Author manuscript; available in PMC: 2014 Jun 16.

Published in final edited form as: Nat Protoc. 2013 Mar 7;8(4):639–651. doi: 10.1038/nprot.2013.024

Table 1. Non-ionic detergents compatible with mass spectrometry of intact membrane protein complexes.

Detergent	Abbreviation	CMC (%)^b
Glucosides
n-Octyl-β-D-glucopyranoside	OG	0.53
n-Nonyl- β -D-glucopyranoside	NG	0.2
Thioglucosides
n-Octyl- β -D-thioglucopyranoside	OTG	0.28
Maltosides
n-Decyl- β -D-maltopyranoside	DM	0.087
n-Dodecyl- β -D-maltopyranoside	DDM	0.0087
n-Undecyl- β -D-maltopyranoside	UDM	0.029
n-Tridecyl- β -D-maltopyranoside	TDM	0.0017
Cymal-5	Cy5	0.12
Cymal-6	Cy6	0.028
Thiomaltosides
n-Dodecyl- β -D-thiomaltopyranoside	DDTM	0.0026
Alkyl Glycosides
Octyl Glucose Neopentyl Glycol	OGNG	0.058
Polyoxyethylene Glycols ^a
Triton X-100	TX100	0.015
C8E4	C₈E₄	0.25
C8E5	C₈E₅	0.25
C10E5	C₁₂E₈	0.031
C12E8	C₁₂E₈	0.0048
C12E9	C₁₂E₉	0.003
Anapoe-58 (Brij-58)	C₁₆E₂₀	0.00045

Depending on the membrane protein, this family of detergents in particular may produce complicated mass spectra.

Critical micelle concentration (CMC) obtained from manufacturer.