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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: J Mol Biol. 2012 Jun 28;423(1):1–13. doi: 10.1016/j.jmb.2012.06.033

Figure 2.

Figure 2

Tol C was selected for study since only 30% of the protein chain is embedded in the membrane. Using a protocol whereby we buffer exchanged the octylglucoside detergent micelle into 1M ammonium acetate solution only a low proportion of the complex survived in the gas phase (upper panel). Considerable dissociation to the monomer was observed and very broad peaks for the trimer, presumably since detergent adhered to the protein subunits. By contrast, when Tol C was introduced from a DDM detergent micelle above the CMC, and when the appropriate MS conditions are employed, a well-resolved trimer is observed with one and two lipid molecules bound (purple and blue symbols).