Table II. List of antibodies used for Western blot analysis including molecular weights, isotypes, clonality, localization, secondary antibodies and concentrations used.

Name	Code	Description	Clonality	Isotype	Concentration	Molecular weight	Cellular localization	Secondary antibody	Additional bands
Anti MSX2	ab69058	Rabbit polyclonal to Msx2/Hox8	polyclonal	IgG	1 μg/mL	29 kDa	nuclear	Goat polyclonal to rabbit IgG at 1/3000 dilution	48, 55
Anti-RUNX2	ab23981	Rabbit polyclonal to RUNX2	polyclonal	IgG	1 μg/mL	57 kDa	nuclear	Goat polyclonal to rabbit IgG at 1/10000 dilution
Anti-Sp7 / osterix antibody	ab22552	Rabbit polyclonal to Sp7/osterix – ChIP grade	polyclonal	IgG	2.5 μg/mL	46 kDa	nuclear	Goat anti-rabbit IgG
Anti-osteopontin antibody	ab91655	Rabbit monoclonal [EPR3688] to osteopontin	monoclonal	IgG	1/1000	35 kDa	secreted	HRP-conjugated goat anti-rabbit at 1/2000 dilution
Anti-osteocalcin antibody	ab13420	Mouse monoclonal [OCG3] to osteocalcin	monoclonal	IgG3	3 μg/mL	12 kDa	secreted	HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution
Anti-actin antibody	ab3280	Mouse monoclonal [ACTN05 (C4)] to actin	monoclonal	IgG1	0.5 μg/mL	42 kDa	cytoplasm > cytoskeleton	HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution