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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Biochem J. 2009 Jun 12;421(1):79–86. doi: 10.1042/BJ20090309

Figure 1. Effect of nitroxides and related compounds on HOCl production by the MPO-H2O2-Cl− system.

Figure 1

Reactions contained MPO (100 nM), Cl− (100 mM), Met (250 μM) and nitroxides and related compounds (0.25–10 μM) and were initiated by the addition of H2O2 (50 μM). In control reactions, nitroxides and related compounds were omitted. Met sulfoxide, a product of the reaction of Met with HOCl, was quantified by HPLC after 30 min. Data are means ± S.E.M., n ≥ 3 independent experiments. IC50 values were determined by fitting a rectangular hyperbola to the dose–response curves using non-linear regression. (A) Effect of nitroxides and related compounds (10 μM). (B) Inhibition by 4-aminoTEMPO (0.25–10 μM). (C) Inhibition by 3-aminoPROXYL (0.25–10 μM).