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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Biochem J. 2009 Jun 12;421(1):79–86. doi: 10.1042/BJ20090309

Figure 3. Nitroxide-mediated inhibition of H2O2 consumption by the MPO-H2O2-Cl− system.

Figure 3

Reactions contained H2O2 (50 μM), Cl− (100 mM), Met (250 μM) and nitroxides (1–500 μM) and were initiated by the addition of MPO (20 nM, indicated by black arrowheads). (A) Inhibition by 4-aminoTEMPO; (a) control (no nitroxide), (b) 1 μM, (c) 10 μM, (d) 50 μM and (e) 500 μM nitroxide. (B) Inhibition by 4-hydroxyTEMPO; legend as for (A). (C) Inhibition by 4-carboxyTEMPO; legend as for (A). Results are representative of multiple independent experiments. Results for other nitroxides are given in Supplementary Figure S4.