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. 2014 Jun 16;7:45. doi: 10.1186/1756-6606-7-45

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Copyright © 2014 Yoshida et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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RP patient’s iPSCs. (A) A colony of RP#5 iPSCs derived from an RP patient’s skin cells. (B) DNA analysis of the rhodopsin gene in the RP#5 iPSCs and control 201B7 iPSCs. (C-E) Immunostaining for pluripotent markers; the nuclei were counterstained with DAPI (blue). (C) Nanog and Oct3/4. (D) Tra-1-60. (E) SSEA4. (F) Teratoma formation assay showing that the RP#5 iPSCs gave rise to all three germ layers, confirming their pluripotency. Scale bar, 1 mm (A), 250 μm (C-F).