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Immunohistochemical determination of CA9 expression. The positivity of a tumor for CA-9 was semi-quantitatively analyzed according to the percentage of cells showing membrane positivity. Score 0, negative staining in all cells; score 1+, weakly positive or focally positive staining in <10% of the cells; score 2+, moderately positive staining covering >10% of the cells; and score 3+, strongly positive staining, including >10% of the cells.
