Figure 2.

Specificity of Pro and Telo by Western Blotting of tissue extracts. A soluble tissue extract of shaved mouse skin was prepared using detergent-free neutral buffer as previously described for lung tissue (Lee et al., 2014). Following centrifugation, the pellet was re-extracted by boiling for 3 min in SDS-PAGE sample buffer. Aliquots of the soluble extract (Sol) and the pellet extract (Plt) representing equal amounts of tissue were then resolved by SDS-PAGE and Western blotted using Pro and Telo as indicated. The identity of the various forms of collagen I recognized by these antibodies is described in the text and indicated on this figure.