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. 2014 May 27;14:143. doi: 10.1186/1471-2229-14-143

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Copyright © 2014 Gillman et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cartoon depiction of the SACPD-C (Glyma14g27990) locus in various radiation and EMS-induced mutant lines. (a) An arrow indicates the predicted transcriptional start sites, dark gray boxes indicate exons, introns are indicated by lines, and 5’- or 3’- untranslated regions are represented by light gray boxes. Regions with amino acid sequence due to mutagenesis are indicated by red lines or blocks. (b) Weblogo SACPD-C amino acid sequence surrounding the residue mutated (V211E) in EMS induced high stearic acid mutant line 194D. The height of letters is proportionate to the degree of conservation among 100 diverse NCBI entries matching SACPD-C. The specific residue affecting by the 194D mutation is indicated by arrow. Image was created using online tool (http://weblogo.berkeley.edu/).