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. 2014 Jul 2;181(100):40–47. doi: 10.1016/j.ijfoodmicro.2014.04.017

Fig. 4.

Fig. 4

Log10 plot of optical density (OD 600 nm) measurement of lag phase in Z. bailii in shake flasks of YEPD pH 4.0 containing 6 mM sorbic acid at 25 °C. a. Data represent the mean and standard deviations of data from experiments carried out in triplicate. b. Lag phase in Z. bailii (log10 plot) measured by haemocytometer (open squares), CFU on agar plates (open circles) or microtitre plates (closed squares) in shake flasks of YEPD pH 4.0 containing 6 mM sorbic acid at 25 °C. Data represent the mean and standard deviations of data from experiments carried out in triplicate. c. Log10 plot of lag phase of Z. bailii in the total population and the resistant sub-population growing in shake flasks of YEPD pH 4.0 containing 6 mM sorbic acid at 25 °C, measured by CFU on agar containing 6 mM sorbic acid (closed squares) or 0 sorbic acid (open squares). Data represent the mean and standard deviations of data from experiments carried out in triplicate. d. Death of the bulk population of Z. bailii during lag phase in shake flasks of YEPD pH 4.0 containing 6 mM sorbic acid at 25 °C. Data comprise the subtraction of the resistant sub-population from the total population, averaged using the agar and microtitre data and representing the mean and standard deviations of experiments carried out in triplicate.