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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: ACS Chem Biol. 2012 Apr 18;7(6):1120–1127. doi: 10.1021/cb300081s

Figure 3.

Figure 3

L7/L12 exchange from ribosomes trapped in the posttranslocational state. A) Low m/z region of a mass spectrum recorded for an equimolar solution of l- and h-ribosome-EF–G complex trapped in the posttranslocational state by fusidic acid, prior to subunit exchange. B–C) The 6+ charge state of (L7/L12)4 stripped complex at time t=0 (lower spectra) and after 3h incubation at 37°C (upper spectra) for ribosomes in: B) the absence of EF–G and C) for the EF–G complex. Peaks corresponding to tetramers with different ratios of (l:h) L7/L12 are highlighted in blue (4:0/0:4), red (3:1/1:3) and green (2:2).