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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Nano Lett. 2010 Jun 9;10(6):2211–2219. doi: 10.1021/nl101157z

FIGURE 3.

FIGURE 3

Characterization of the vesicles containing NPs after endocytosis. (a) Confocal micrographs of LN-229 cells transiently transfected with the lysosomal marker, LAMP1-GFP, following incubation with PEI–PEG functionalized NPs. Images show representative cells 10 min (top), 3 h (middle), and 20 h (bottom) after incubation at 37 °C. (b) Confocal micrographs of LN-229 cells after overnight endocytosis of streptavidin functionalized NPs via surface biotinylation. The endolysosomal compartment was stained with LysoTracker Blue. All scale bars are 20 μm.