Table 1. Summary of the electrophysiological properties of layer V pyramidal neurons.
| Control < 4 hrs (n = 10) | 1–3 hrs (n = 12) | 5–8 hrs (n = 7) | 15–24 hrs (n = 9) | 26–41 hrs (n = 9) | |
|---|---|---|---|---|---|
| Resting membrane potential (mV) | −63 ± 2 | −65 ± 2 | −64 ± 1 | −64 ± 1 | −63 ± 1 |
| Input resistance (MΩ) | 176 ± 29 | 165 ± 18 | 163 ± 27 | 179 ± 21 | 145 ± 19 |
| Membrane time constant (ms) | 25 ± 4 | 28 ± 3 | 24 ± 7 | 25 ± 2 | 23 ± 3 |
| Resonance frequency (Hz) | 2 ± 0.3 | 2 ± 0.2 | 1.7 ± 0.3 | 1.5 ± 0.3 | 1.6 ± 0.3 |
| First spike Amplitude (mV) | 97 ± 5 | 97 ± 2 | 94 ± 4 | 93 ± 3 | 92 ± 3 |
| Half-width spike amplitude (ms) | 2.4 ± 0.2 | 2.5 ± 0.2 | 2.3 ± 0.1 | 2.6 ± 0.2 | 2.2 ± 0.1 |
| mEPSC's frequency (Hz) | 2.2 ± 0.3 | 1.75 ± 0.3 | 2.3 ± 0.4 | 1.5 ± 0.2 | 2.2 ± 0.3 |
| mEPSC's amplitude (pA) | −7.3 ± 0.5 | −6.4 ± 0.5 | −7.8 ± 0.5 | −5.7 ± 0.4 | −8 ± 0.7 |
Basic electrophysiological parameters were monitored during different incubation time in the Braincubator. Values are means ± S.E.M. No significant differences were detected between groups (one-way ANOVA). The first group on the left describes the same electrophysiological basic parameters recorded from neurons incubated under control conditions (<3 hours) for comparison.