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. 2014 Jun 16;8:142. doi: 10.3389/fnins.2014.00142

Figure 1.

Figure 1

Intracerebroventricular infusion of histamine triggers neuronal commitment in the SVZ. (A) Design of the 3 weeks experiment, consisting in 3-day BrdU treatment (twice daily with 12 h interval) starting at the day after surgery. Animals were sacrificed 3 weeks after surgery. (B) Representative fluorescent confocal digital images of BrdU (green; upper panel), Ki67 (green; lower panel) and DCX (red) positive cells observed in the SVZ of control and histamine treated animals in vivo, 21 days after pump installation. Scale bar = 20 μm. Arrows highlight double BrdU+DCX+ (upper panel) or Ki67+DCX+ (lower panel) positive cells. Hoechst staining (blue) labels cell nuclei. (C) From left to right: bargraphs represent the total DCX+ positive cells, total BrdU+DCX+ double positive cells and total Ki67+DCX+ double positive cells in both control and histamine in vivo treated animals, 21 days after pump installation. Ctrl: Control; Hist: Histamine. Data are expressed as mean ± SEM (n = 5–7 mice; *p < 0.05; ***p < 0.001). Statistical analysis was performed using Student's unpaired t-test.