Table 1.

Problems the PE multipositional de Bruijn graph solves in comparison with the conventional de Bruijn graph

Problem	Consequence	Solution
Repeat sequences	Branched paths	Distinguishes paths for repetitive regions by incorporating PE read and alignment position information
Sequencing errors	False-positive paths	Corrects paths from erroneous reads with correct reads aligned to the same position
Low sequencing depth	Incomplete paths	Builds paths from reads in low coverage areas supported by reference