Table 3.
The inhibitory effect of anti-CD137 blocking antibody (10 μg/mL) (a137), 10 -6 M methylprednisolone (MP) alone and in combination on granzyme B, IFNγ and TNFα expression by CD28null(28-) and CD28 + (28+) CD8+ NKT-like cells and NK cells following stimulation of PBMC from COPD patients (mean ± SEM, mean of 8 experiments) compared with control antibody
| |
Granzyme B |
IFNγ |
TNFα |
||||||
|---|---|---|---|---|---|---|---|---|---|
| |
CD8 + NKT-like |
NK |
CD8 + NKT-like |
NK |
CD8 + NKT-like |
NK |
|||
| 28- | 28+ | 28- | 28+ | 28- | 28- | ||||
|
a137 |
21 ± 2* |
14 ± 2 |
14 ± 2 |
26 ± 3* |
20 ± 3 |
22 ± 3 |
30* ± 4 |
23* ± 4 |
24 ± 3 |
|
MP |
1 ± 1 |
3 ± 2 |
2 ± 2 |
2 ± 1 |
5 ± 6 |
43 ± 3^ |
2 ± 1 |
2 ± 1 |
47 ± 9^ |
| a137 + MP | 22 ± 4# | 17 ± 3# | 15 ± 3# | 28 ± 3# | 25 ± 2# | 66 ± 3 | 32 ± 4# | 25 ± 4# | 70 ± 7# |
*The percentage inhibition of granzyme B, IFNγ and TNFα by CD28- and CD28+ NKT-like and NK cells compared with control (same specimen) without treatment/s.
The inhibitory effect of anti-CD137 was greater than for MP for granzyme B, IFNγ and TNFα in all NKT-like cell subsets (p < 0.05 for all).
*The inhibitory effect of anti-CD137 was greater than for the CD28nullCD8+ NKT-like subset than for the CD28 + CD8+ NKT-like subset for granzyme B, IFNγ and TNFα (p < 0.05 for all). ^The inhibitory effect of MP on IFNγ and TNFα by NK cells was significantly greater than for Granzyme B (p < 0.05) and significantly greater than the effect of anti-CD137.
#The combination of anti-CD137 and 10-6 M methylprednisolone resulted in the additive inhibition of granzyme B, IFNγ and TNFα expression in CD28null and CD28+ CD8+ NKT-like cells and NK cells.