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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Nov 19;52(52):13975–13979. doi: 10.1002/anie.201305882

Figure 3.

Figure 3

The influence of the PCC agents on the enzymatic activity of active Akt2. A) The activity of Akt2 directly influences the phosphorylation of the GSK-3α/β peptide (fused with glutathione S-transferase) at Ser9/Ser21. The level of phosphorylated GSK-3α/β thus provides a readout for the activation or inhibition of Akt2 by a molecular probe. B) Western blot analysis of p-GSK-3α/β levels shows that, relative to the DMSO control, both the N-tL and the commercial anti-pS473 antibody activate Akt above baseline. The C-tL inhibits Akt2 kinase activity. (A=adenosine; P=phosphate).