Scheme 1.
The preparation of 1; a divalent zinc chelator presenting both biotin and azide moieties. Complex 1 is comprised of 1 bonded to the pSer474 of the 32 amino acid peptide fragment of Akt2. The graphic of complex 1 (bottom right; constructed using PYMOL[18] and ChemBio3D Ultra) illustrates the scale of 1 (purple) bonded to pS474 on the polypeptide (blue).