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Plasmid construction for recombinant PPRV rescue. (A) pN75/1-insertion was constructed previously [22] through insertion of a morbillivirus gene start (GS) sequence, Not I and Pme I sites, gene end (GE) sequence, and CTT intergenic trinucleotides between the P and M genes of genomic PPRV cDNA. (B) The VP1 ORF with a Kozak sequence at the 5' end of the ORF was inserted into plasmid pN75/1-insertion to generate plasmid pN75/1-VP1.
