TABLE 2.
Oligonucleotide primers used in this study
| Number | Sequence (relevant restriction sites underlined, 5′–3′ |
|---|---|
| 1 | AAATCTAGATCATCTCGTAAAAGTCAGACCG (XbaI site underlined) |
| 2 | TTTGATATCATTGGCCAAACATACTTCATTG (EcoRV site underlined) |
| 3 | CCGGATATCGCTTAATTTTTTAGGACAATTC (EcoRV site underlined) |
| 4 | TTTCTCGAGAACCGAGTCTTGGTTTAGCGCC (XhoI site underlined) |
| 5 | AAATCTAGAGGCATTCAGGATTTTAATCCC (XbaI site underlined) |
| 6 | TTTGATATCATGAATTCTCAAACAATAGAAC (EcoRV site underlined) |
| 7 | AAAAAGCTTAAATTTGCGTTTGATCATTACC (HindIII site underlined) |
| 8 | TTTCTCGAGGACCACCTCGATCGTCGCCG (XhoI site underlined) |
| 9 | TTTTCTAGAACCGAGTCTTGGTTTAGCGCC (XbaI site underlined) |
| 10 | AAACCATGGCTGACCCCAACCCTTGTAATG (NcoI site underlined) |
| 11 | TTTGGATCCTTAAGCCGATTGCACCGAATC (BamHI site underlined) |
| 12 | TGTTTGGCCACATATGGGATGGAGCCACCCGCAGTTCGAAAAAGGCGCCATGACTGACCCCAACCCTTGTAATG (NdeI site underlined) |
| 13 | AAAAGGATCCTTAAGCCGATTGCACCGAATC (BamHI site underlined) |
| 14 | CCGGAATTCTAAAAACCTCATTGATTTAC (EcoRI site underlined) |
| 15 | ATACCATGGTTAATTAACAGGAGAATC (NcoI site underlined) |