FIGURE 4.

Effect of pep5-cpp in HeLa cells. A, HeLa cells were treated with peptide 5, cpp alone, or SCB for 24 h. B, cells were incubated with pep5-cpp at different concentrations (1, 10, 25, 50, 75 and 100 μm) for 24 h. C, cells were treated with pep5-cpp and SCB (control) for 10 h at 50 and 100 μm. After all tests, the cells were fixed in 70% ethanol and analyzed by propidium iodide staining and flow cytometry. Results were considered significant when p was ≤0.001 (***) or ≤0.05 (#). The data shown here are representative of three experiments performed in triplicate. NT, not treated; pep-cpp, cpp fused to the C terminus of the peptides; cpp-pep, cpp fused to the N terminus of the peptides. Error bars, S.E.