FIGURE 6.

Effect of modifications in the original sequence of pep5-cpp. A, effect of deletions in pep5 sequence. Cells were treated with pep5-cpp, SCB (control), and ΔN- or ΔC-pep5-cpp (pep5 with deletions) for 24 h (100 μm) and then analyzed by flow cytometry. NT, not treated, SCB control; ΔN-pep5-cpp, deletion of one amino acid at the N terminus; A, B, C, and D, C terminus deletions of 1, 2, 3, and 4 amino acids, respectively. B, analysis of substitutions in the original sequence of pep5. Cells were treated with pep5-cpp, SCB, and a modified pep5-cpp for 24 h (100 μm) and then analyzed by flow cytometry. A, B, and C, pep5 with one or more substitutions, in the original sequence, for alanine. C, cells were treated with pep5-cpp (100 μm) and the acetylated pep5-cpp at different concentrations (1, 10, 50, and 100 μm) for 24 h and then analyzed by flow cytometry. Ac-pep5-cpp, acetylated pep5. The data shown here are representative of three experiments performed in triplicate, and the results were considered significant when p was <0.001 (***). Error bars, S.E.