TABLE 3.
Oligosaccharide substrate specificity of PatBSUMO
Potential substrates in 50 mm sodium phosphate buffer, pH 6.5, containing 4 mm pNP-Ac were incubated with 1.0 μm PatBSUMO, and samples of reaction mixtures were subjected to MALDI-TOF MS analysis. ND, not detected.
| Potential substrate |
m/z [m + Na] of potential O-acetylated product |
Difference | |
|---|---|---|---|
| Theoretical | Detected | ||
| m/z | m/z | ||
| PG metabolites | |||
| GlcNAc | 286.09 | ND | |
| MurNAc | 316.10 | ND | |
| GlcNAc-MurNAc | 561.19 | ND | |
| GlcNAc-MurNAc-dipeptide | 759.28 | ND | |
| Chitooligosaccharides | |||
| GlcNAc2 | 489.17 | ND | |
| GlcNAc2-O-benzyl | 579.22 | 579.45 | 0.23 |
| GlcNAc3 | 692.25 | 692.45 | 0.20 |
| GlcNAc4 | 895.33 | 894.54 | −0.79 |
| GlcNAc5 | 1,098.41 | 1098.85 | 0.44 |
| GlcNAc6 | 1,301.49 | 1302.18 | 0.69 |
| Other oligosaccharides | |||
| β-1,4 Glc6 | 1,055.33 | ND | |
| β-1,4 Glc4 | 731.22 | ND | |
| α-1,4 Glc3 | 569.17 | ND | |
| Xylose6 | 875.26 | ND | |
| Xylose4 | 611.18 | ND | |
| GlcN6 | 1,049.42 | ND | |