TABLE 5.
Muropeptide products of PatBSUMO activity on PG enriched with muro-tripeptides identified by ESI - ion trap MS
PG digested with lysozyme, E. coli PBPs 4 and 7, and H. pylori Csd6 in 50 mm sodium phosphate buffer, pH 7.0, was incubated with 4 mm pNP-Ac in the absence and presence of 1 μm PatBSUMO and subjected to MS analysis. Muropeptides listed in black were present in both reaction product mixtures, whereas those in red were unique to reactions in the presence of PatBSUMO.
