FIGURE 1.

Identification of mutant mice by PCR analysis and KCNE3 protein in SGNs of Kcne3 null mice. Tail DNA was amplified with primers spanning the NeoR insertion site, and the wild-type and mutant chromosomes yield products of 204, 502, and 260 bp, respectively. Localization of KCNE3 in cultured SGNs (stained in green) isolated from P12 Kcne3^+/+ and Kcne3^−/− mice showed no reactivity toward a specific antibody directed against the protein. No positive reactivity was observed in 10 different samples from 10 different Kcne3^−/− SGNs. In contrast, as shown, wild-type SGNs had positive reactivity toward the same antibody. SGNs were stained with antibodies against the neuronal marker, Tuj1 (in cyan), and the nuclei were stained with DAPI (in blue). The right panel is the merged image (scale bar, 10 μm).