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. 2014 Apr 15;289(24):16855–16865. doi: 10.1074/jbc.M114.564005

FIGURE 4.

FIGURE 4.

Two-dimensional EM analysis of nNOS-CaM cross-linked with glutaraldehyde. A, nNOS-CaM cross-linked (CXL) with 0.01% glutaraldehyde is shown in a representative micrograph image and selected particles (scale bar = 200 Å). B, two-dimensional reference-free averages of nNOS-CaM CXL with the number of single particles per average (scale bar = 100 Å). C, comparison of two-dimensional averages of uncross-linked nNOS-CaM in the closed conformation and nNOS-CaM CXL, following two-dimensional alignment.