FIGURE 5.

RCT initial model generation and analysis of the final reconstruction. A, two-dimensional reference-free class averages of cross-linked nNOS-CaM from untilted images with the indicated classes used in the RCT reconstructions. B, three-dimensional RCT reconstructions, generated from tilted data corresponding to the indicated two-dimensional class. The reconstruction from class 13 was used as the starting model for additional three-dimensional refinement. C, two-dimensional projections (Proj.) of the final three-dimensional reconstruction aligned to reference-free class averages (RF Av.) of cross-linked nNOS-CaM. D, angle distribution image from the final round of refinement with a 7.5° increment, generated with XMIPP (48).