TABLE 1.

Steady-state kinetics of incorporation of dCTP, dATP, and dGTP opposite G and 8-oxoG by hpol η

Template base	dNTP	kcat	Km	kcat/Km	fa
		s−1	μm	μm−1 s−1
G	dCTP	1.33 ± 0.05	1.3 ± 0.2	1.0 ± 0.16
G	dATP	0.10 ± 0.01	92 ± 23	0.0011 ± .0003	0.001
G	dGTP	0.15 ± 0.02	80 ± 34	0.0019 ± 0.0008	0.0019
8-OxoG	dCTP	1.20 ± 0.03	2.3 ± 0.2	0.52 ± 0.05
8-OxoG	dATP	0.78 ± 0.03	5.4 ± 0.6	0.15 ± 0.02	0.28
8-OxoG	dGTP	0.43 ± 0.02	27 ± 5	0.016 ± 0.003	0.031

^a f = (k_cat/K_m)_dNTP/(k_cat/K_m)_dCTP.