Caspase-3/7 activity. The caspase-3/7 activity assay was performed 6, 12, and 24 h after apoptosis and 6, 12, 24, 48, 72, 96, 120, 144, and 168 h after differentiation induction. (The data are expressed relative to control). The data are means ± S.D. (error bars) of triplicate experiments.