Apoptosis detection using annexin V/PI staining. Viable cells (annexin V−/PI−), early apoptotic cells (annexin V+/PI−), late apoptotic cells (annexin V−/PI+), and necrotic cells (annexin V+/PI+) are located in the bottom left, bottom right, top right, and top left quadrants, respectively. The numbers in each quadrant represent the percentage of cells.