TABLE 1.
Gating kinetics of mutated BK channels
The first column shows the mutated BK type channels. The activation (τact) and deactivation (τdea) time constants were calculated at 100 mV except for mSlo1(K392E,R393D)-β1 and mSlo1(K392E,R393D)-β1(E13K,T14K), which were calculated at 200 mV, respectively.
| BK channel | τact | τdea |
|---|---|---|
| ms | ms | |
| mSlo1-β1 | 2.4 ± 0.4 | 10 ± 0.8 |
| mSlo1-β1(E13K,T14K) | 4.0 ± 0.6 | 4.5 ± 0.6 |
| mSlo1(K392E,R393D)-β1 | 4.2 ± 0.7 (200 mV) | 0.78 ± 0.2 (200 mV) |
| mSlo1(K392E,R393D)-β1 (E13K,T14K) | 3.8 ± 0.4 (200 mV) | 0.85 ± 0.2 (200 mV) |
| mSlo1-β1(L5Q,V6Q,M7Q) | 7.4 ± 0.8 | 5.2 ± 0.5 |
| mSlo1(L906Q,L908Q)-β1 | 15 ± 1.5 | 5.0 ± 0.4 |
| mSlo1(L906Q,L908Q)-β1 (L5Q,V6Q,M7Q) | 60 ± 3.4 | 1.4 ± 0.2 |
| mSlo1-β1(K3Q,K4Q) | 2.4 ± 0.3 | 7.4 ± 0.8 |
| mSlo1(5D5N)-β1 | 13 ± 1.0 | 1.7 ± 0.3 |
| mSlo1(5D5N)-β1(K3Q,K4Q) | 30 ± 2.4 | 2.3 ± 0.4 |