TABLE 2.
Influence of mutations on BK channels
The first column shows the corresponding mutations of BK channels. Estimates for the values of V50 and Z for the different mutations were obtained by fitting G-V curves to the Boltzmann equation. The free energy is given by −ZFV50.
| BK channel | V50 | Z | −ZFV50 |
|---|---|---|---|
| mV | kcal/mol | ||
| mSlo1-β1 | −46.7 ± 4.8 | 1.70 ± 0.21 | 1.83 ± 0.02 |
| mSlo1-β1(E13K,T14K) | 0.7 ± 3.2 | 2.07 ± 0.17 | 0.03 ± 0.01 |
| mSlo1(K392D,R393D)-β1 | 178.3 ± 6.2 | 1.16 ± 0.15 | −4.79 ± 0.02 |
| mSlo1(K392D,R393D)-β1(E13K,T14K) | 184.2 ± 6.5 | 1.17 ± 0.16 | −4.97 ± 0.02 |
| mSlo1-β1(L5Q,V6Q,M7Q) | −7.2 ± 5.5 | 1.70 ± 0.15 | 0.28 ± 0.02 |
| mSlo1-β1(L5Q) | −33.5 ± 5.2 | 1.70 ± 0.13 | 1.32 ± 0.02 |
| mSlo1-β1(V6Q) | −18.5 ± 5.1 | 1.69 ± 0.14 | 0.71 ± 0.02 |
| mSlo1-β1(M7Q) | −20.4 ± 4.9 | 1.70 ± 0.15 | 0.80 ± 0.02 |
| mSlo1(L906Q,L908Q)-β1 | 46.7 ± 6.1 | 1.74 ± 0.20 | −1.88 ± 0.02 |
| mSlo1(L906Q,L908Q)-β1(L5Q,V6Q,M7Q) | 127.9 ± 6.4 | 1.64 ± 0.17 | −4.86 ± 0.02 |
| mSlo1-β1(L5A,V6A,M7A) | −7.0 ± 5.6 | 2.03 ± 0.22 | 0.33 ± 0.02 |
| mSlo1(L906A,L908A)-β1 | 128.0 ± 6.2 | 1.24 ± 0.15 | 3.68 ± 0.02 |
| mSlo1(L906A,L908A)-β1(L5A,V6A,M7A) | 145.1 ± 6.1 | 1.16 ± 0.16 | 3.88 ± 0.02 |
| mSlo1-β1(K3Q,K4Q) | −35.1 ± 4.7 | 1.60 ± 0.15 | 1.3 ± 0.01 |
| mSlo1(5D5N)-β1 | 59.1 ± 4.9 | 1.51 ± 0.16 | −2.07 ± 0.02 |
| mSlo1(5D5N)-β1(K3Q,K4Q) | 62.0 ± 6.1 | 1.44 ± 0.07 | −2.08 ± 0.01 |
| mSlo1(D362R,D367R)-β1 | 6.49 ± 5.0 | 1.74 ± 0.15 | −0.26 ± 0.02 |
| mSlo1(D362R,D367R)-β1(K3Q,K4Q) | 18.3 ± 5.5 | 1.78 ± 0.13 | −0.75 ± 0.02 |
| mSlo1(L110Q)-β1 | −63.2 ± 6.5 | 1.70 ± 0.08 | 2.48 ± 0.02 |
| mSlo1(L110Q)-β1(L5Q,V6Q,M7Q) | −18.6 ± 5.1 | 2.02 ± 0.14 | 0.87 ± 0.02 |
| mSlo1(V114Q)-β1 | −13.2 ± 6.0 | 1.74 ± 0.08 | 0.53 ± 0.02 |
| mSlo1(V114Q)-β1(L5Q,V6Q,M7Q) | 38.4 ± 5.8 | 1.59 ± 0.12 | −1.41 ± 0.02 |
| mSlo1(V117Q)-β1 | −81.2 ± 6.5 | 1.52 ± 0.14 | 2.86 ± 0.20 |
| mSlo1(V117Q)-β1(L5Q,V6Q,M7Q) | −45.6 ± 5.3 | 1.74 ± 0.10 | 1.84 ± 0.01 |
| mSlo1(L118Q)-β1 | −70 ± 5.2 | 1.82 ± 0.13 | 2.94 ± 0.02 |
| mSlo1(L118Q)-β1(L5Q,V6Q,M7Q) | −21.3 ± 5.5 | 1.68 ± 0.09 | 0.83 ± 0.01 |
| mSlo1(F120Q)-β1 | −37.5 ± 4.8 | 1.67 ± 0.14 | 1.45 ± 0.02 |
| mSlo1(F120Q)-β1(L5Q,V6Q,M7Q) | 11.7 ± 5.6 | 1.78 ± 0.12 | −0.48 ± 0.02 |
| mSlo1(I215Q)-β1 | −18.2 ± 6.5 | 1.74 ± 0.06 | 0.73 ± 0.01 |
| mSlo1(I215Q)-β1(L5Q,V6Q,M7Q) | 35.4 ± 4.5 | 1.62 ± 0.10 | −1.33 ± 0.01 |
| mSlo1(F217Q)-β1 | −78.7 ± 6.4 | 1.84 ± 0.11 | 3.36 ± 0.02 |
| mSlo1(F217Q)-β1(L5Q,V6Q,M7Q) | −38.5 ± 5.2 | 1.86 ± 0.12 | 1.65 ± 0.02 |
| mSlo1(I220Q)-β1 | −186.8 ± 6.8 | 0.85 ± 0.22 | 3.66 ± 0.03 |
| mSlo1(I220Q)-β1(L5Q,V6Q,M7Q) | −152.2 ± 5.7 | 0.78 ± 0.15 | 2.73 ± 0.02 |
| mSlo1(F223Q)-β1 | −135.1 ± 5.5 | 0.95 ± 0.12 | 2.97 ± 0.02 |
| mSlo1(F223Q)-β1(L5Q,V6Q,M7Q) | −71.5 ± 5.6 | 1.08 ± 0.11 | 1.78 ± 0.02 |
| mSlo1(L235Q)-β1 | 41.5 ± 4.9 | 1.12 ± 0.13 | −1.07 ± 0.02 |
| mSlo1(L235Q)-β1(L5Q,V6Q,M7Q) | 74 ± 6.5 | 1.54 ± 0.09 | −2.64 ± 0.02 |
| mSlo1(L239Q)-β1 | −21.7 ± 5.4 | 1.62 ± 0.12 | 0.81 ± 0.02 |
| mSlo1(L239Q)-β1(L5Q,V6Q,M7Q) | 35.2 ± 6.1 | 1.62 ± 0.08 | −1.32 ± 0.01 |
| mSlo1(V339Q)-β1 | −47.8 ± 4.8 | 1.70 ± 0.13 | 1.88 ± 0.01 |
| mSlo1(V339Q)-β1(L5Q,V6Q,M7Q) | −6.7 ± 4.5 | 1.70 ± 0.10 | 0.26 ± 0.01 |
| mSlo1(L353Q)-β1 | −35.5 ± 5.1 | 1.82 ± 0.12 | 1.49 ± 0.02 |
| mSlo1(L353Q)-β1(L5Q,V6Q,M7Q) | −4.7 ± 4.6 | 1.82 ± 0.13 | 0.2 ± 0.01 |
| mSlo1(L381Q)-β1 | −45.5 ± 4.8 | 1.70 ± 0.09 | 1.79 ± 0.01 |
| mSlo1(L381Q)-β1(L5Q,V6Q,M7Q) | −5.1 ± 5.0 | 1.68 ± 0.12 | 0.20 ± 0.02 |
| mSlo1(L385Q)-β1 | 50.1 ± 5.7 | 1.66 ± 0.13 | −1.93 ± 0.02 |
| mSlo1(L385Q)-β1(L5Q,V6Q,M7Q) | 94.5 ± 6.5 | 1.45 ± 0.15 | −3.18 ± 0.02 |
| mSlo1(I517Q,I519Q,L529Q)-β1 | 12.2 ± 5.5 | 1.72 ± 0.12 | −0.49 ± 0.02 |
| mSlo1(I517Q,I519Q,L529Q)-β1(L5Q,V6Q,M7Q) | 59.1 ± 5.5 | 1.72 ± 0.11 | −2.37 ± 0.02 |
| mSlo1(I813Q)-β1 | −56.3 ± 5.7 | 1.68 ± 0.12 | 2.19 ± 0.02 |
| mSlo1(I813Q)-β1(L5Q,V6Q,M7Q) | −11.5 ± 6.1 | 1.65 ± 0.13 | 0.44 ± 0.02 |
| mSlo1(F893Q)-β1 | 18.5 ± 5.4 | 1.71 ± 0.09 | −0.73 ± 0.01 |
| mSlo1(F893Q)-β1(L5Q,V6Q,M7Q) | 66.5 ± 6.5 | 1.69 ± 0.13 | −2.60 ± 0.02 |